THREATS TO CANNABIS GROWTH

There are a variety of naturally occurring airborne microorganisms that can cause plants to develop mold or a powdery mildew, rendering them worthless. Standard HVAC systems can actually increase this threat as these damp and dark systems offer a perfect breeding ground for bacterial growth. Worse still, ventilation systems are very efficient at spreading microscopic spores throughout a building. 

Air filtration systems can keep the indoor growing area free of airborne fungal spores. Botrytis cinerea spores (commonly known as gray mold) are approximately 11-12 microns in diameter (Gull 1971) and this size spore will be nearly completely removed by filters rated MERV 12 and higher. Even dust filters of the lowest rating (MERV 6-8) may remove 50 percent of spores from the air and, with multiple passes (via recirculated air), spores may be completely removed (Kowalski 2006 & 2009).

While air filtration provides protection against airborne spores, it has a downside for agricultural production. Humidity levels necessary for cannabis plants to thrive can cause filter media to become damp, encouraging the captured bacteria to proliferate.

The Botrytis cinerea spores hail from the outdoors and are brought into indoor cultivation rooms by shoes and clothing. They are also found as common contaminants in about half of indoor environments in the USA, with mean concentrations of about 45-49 CFU/m³ (Shelton 2002).

It is therefore important that workers clean and decontaminate themselves prior to entering a sterile cultivation environment. Normal disinfection procedures, such as those used in hospital environments, can be adapted for cultivation rooms.

Other methods to control Botrytis cinerea include traditional fungicides, but some of these methods may leave hazardous residues that are not suitable for consumption by seriously ill patients (Cervantes 2006). Organic methods are also being studied, including the use of competing or parasitical microorganisms (Wu 2013, Schumacher 2011, Costa 2013).

The air quality inside cultivation rooms can also be controlled using air filtration and positive pressurization, while interior disinfection can be accomplished with chemicals like bleach.

Although the aforementioned methods can be effective in combating microbial threats to cannabis production, the cleanest, most direct way of inactivating them is to use ultraviolet germicidal irradiation (UVGI).

UVGI IN CULTIVATION FACILITIES 

Ultraviolet germicidal energy or light energy in the UV-C wavelength (253.7nm) disassociates molecular bonds, which in turn disinfects and disintegrates organic materials, making it an ideal means of permanently inactivating microbes like Botrytis cinerea.

In fact, scientists have yet to find a microorganism that’s totally immune to the destructive effects of UV-C, including superbugs and all other antibiotic-resistant microbes.

There are two approaches to using UV-C to control mold and bacterial proliferation in indoor cannabis gardens: No. 1, UV-C air-stream disinfection systems, and No. 2, UV-C surface disinfection systems.

Air-Stream Disinfection

Airstream disinfection systems employ UV-C lamps to target pathogens from outdoor and/or return air, which contain airborne pathogens. Kill ratios over 99.9 percent on a first-pass basis have been modeled and, as air is re-circulated, concentrations are further reduced by each subsequent pass or multiple dosing.

HVAC Surface Cleaning

Surface-cleaning UV-C systems provide 24/7 irradiation of HVACR components to destroy bacteria, viruses, and mold that settle and proliferate on coils, air filters, ducts, and drain pans. UV-C prevents these areas from becoming microbial reservoirs for pathogen growth that will eventually spread into air streams. They also can provide first pass kill ratios of airborne pathogens of up to 30 percent, with ancillary benefits of restored cleanliness, heat exchange efficiency, and energy use (ASHRAE 2011- Fencl 2013/2014).

Latorre et al (2012) tested the effectiveness of UV-A, UV-B, and UV-C irradiation on Botrytis cinerea and found UV-C to be the most effective spectrum. The study employed a Philips model F15T8BLB with 20 W of UV output. Results indicated zero survival of conidia (spores) at the highest UV dose of 1100 J/m².

Mercier et al (2001) used UV-C irradiation in the range of 400-4500 J/m² with a General Electric G30T8 UV lamp (30 W of UV output) to control the decay caused by Botrytis cinerea on Bell peppers. Spore germination was inhibited both in vivo and in vitro with spore survival rates of 0-5 percent after a total UV dose of 4400 J/m².

Marquenie (2002) irradiated Botrytis cinerea spores using five fluorescent UV lamps of 8W UV output each, and the results show a single stage curve up to the maximum tested dose of 1000 J/m². The first stage of decay, which is often the most important in disinfection applications, varies from k_f = 0.0022 – 0.0093 m²/J. The mean value for the first stage decay constant of Botrytis cinerea is therefore k = 0.0052 m²/J. The range of D90 values, or the dose required for 90 percent disinfection of spores is approximately 440-2000 J/m². This range of UV susceptibilities compares well with other fungal spores such as Aspergillus or Penicillium (Kowalski 2009).

A critical part in the design of indoor cultivation rooms for medical marijuana is the control of airborne fungal spores, and although most existing facilities are poorly suited to prevent the ingress of fungal spores, they can usually be retrofitted with air filters, fans, and UV systems to render them relatively free of spores. It is important, however, that the facility be air tight and under positive pressure to maintain the high levels of cleanliness necessary to avoid outbreaks of mold. And ideally, every cultivation center should have a pressurized anteroom in which worker access will not cause spores to enter from the outdoors. Operating protocols and regular decontamination are necessary to ensure high levels of cleanliness and disinfection.

CASE-IN-POINT: ACCELERATED GROWTH SOLUTIONS 

Conor Guckian, a 20-year veteran of HVAC equipment sales, founded Sacramento-based Accele rated Growth Solutions to provide climate and environmental control equipment for the budding cannabis industry.

“Legalization of cannabis has made entrepreneurs more deliberate in the way they design their cultivation facilities,” said Guckian. “Cultivators are building facilities on a much grander scale, with long-term performance in mind, and are increasingly investing in technologies that will maximize their total yields.”

Guckian’s Accelerated Growth (AG) systems are designed to address these rising demands by regulating temperature, humidity, and vapor pressure differentials to meet the plants’ needs during each step of development.

“Our AG systems modulate to maintain precise temperature and humidity no matter what the load in the space is and, in so doing, provide an environment for plants to flourish,” said Guckian.

One standard, built-in feature in all the AG systems is UV-C germicidal irradiation from Santa-Clarita, California-based UV Resources. The 253.7 nm C-band wavelength inactivates virtually all microorganisms that form on HVACR cooling coils. Left unchecked, these microbes might cause molds and powdery mildew to form, ruining entire crops.

“Placing UV-C lamps directly over the cooling coils is crucial to our ability to maintain a healthy, successful facility,” said Guckian. “An added benefit is the disinfected condensate from the cooling coil that can be recycled for use elsewhere in the facility.”

Alternatives to UV-C include mechanical cleanings, which are often cumbersome and difficult in a crowded facility, or the use of pesticides, which are increasingly avoided by growers due to the hazard they pose to many medical marijuana patients. According to Guckian, UV-C provides the simplest, most efficient means of protecting a cannabis crop from mold.

He points to one cultivation facility based in Desert Hot Springs, California, as an example. The 40,000 square foot facility had previously been using stand-alone dehumidifiers, coupled with air conditioners, to control its growing environment. Not only was this existing setup unable to effectively modulate and maintain an ideal growing environment, but the absence of UV-C disinfection meant that mold was always a looming threat.

To improve yields, the facility installed 12, 35-ton AG environmental units, each serving a 70-light production room.

“Installation is incredibly simple,” said Guckian. “Units are mounted on the floor outside the light room. A small hole is cut in the wall for the return air, and the supply air is released into a fabric duct system.”

After seven months of operation, the facility has increased its yields about 60 percent; from two pounds per light to roughly 3.25 pounds per light.

“It’s a relatively inexpensive protection for an incredibly expensive crop,” said Guckian. “You shouldn’t even build a facility without it. It’s not even a question.”

Publication date: 8/06/2018

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